Alan M. Kleinfeld

BIOPHYSICS | Adjunct Member
Phone: (858) 597-3724 | Fax: (858) 597-3804 |
Alan M. Kleinfeld

Free fatty acids (FFA) provide the major source of the body’s long term energy needs and are therefore essential for health. At the same time, disruption of the normal regulation of FFA levels by diseases such as diabetes, cancer, liver diseases and cardiovascular disease can result in serious consequences including death. The focus of our laboratory is to determine how free fatty acids are transported across cell membranes and how the levels and types of FFA change in disease. To carry out these studies we have developed molecular sensors that allow FFA to be visualized within living cells and allow the profiling of blood levels of FFA for the diagnosis of major diseases. Results from these studies raise the possibility of new therapeutic strategies for treating breast cancer.

BIOPHYSICS | Adjunct Member
Phone: (858) 597-3724 | Fax: (858) 597-3804 |
Alan M. Kleinfeld

Free fatty acids (FFA) provide the major source of the body’s long term energy needs and are therefore essential for health. At the same time, disruption of the normal regulation of FFA levels by diseases such as diabetes, cancer, liver diseases and cardiovascular disease can result in serious consequences including death. The focus of our laboratory is to determine how free fatty acids are transported across cell membranes and how the levels and types of FFA change in disease. To carry out these studies we have developed molecular sensors that allow FFA to be visualized within living cells and allow the profiling of blood levels of FFA for the diagnosis of major diseases. Results from these studies raise the possibility of new therapeutic strategies for treating breast cancer.

Biography

Education

  • 1968: Ph.D Rutgers University, New Brunswick, NJ (physics)
  • 1963: B.A. University of Wisconsin, Madison, WI

Positions

  • 1998-present: Member, Torrey Pines Institute for Molecular Studies, San Diego, CA
  • 1987-1998: Member, Medical Biology Institute, La Jolla, CA
  • 1983-1987: Associate Professor of Physiology and Biophysics, Department of Physiology and Biophysics, Harvard Medical School
  • 1981-1983: Assistant Professor of Biophysics, Department of Physiology and Biophysics, Harvard Medical School
  • 1979-1987: Faculty, the program in Cell and Developmental Biology, Harvard Medical School
  • 1977-1981: Lecturer on Biophysics, Biophysical Laboratory, Harvard Medical School
  • 1973-1977: Privat Dozent, Physics Institute, University of Cologne
  • 1973: Admitted to the faculty (Habilitation), Physics Institute, University of Cologne, Cologne, Germany
  • 1968-1973: Group Leader, Physics Institute, University of Cologne

Professional Affiliations

  • 1998-present: President/CEO, FFA Sciences LLC, San Diego, CA
  • 2010: Member, Advisory Committee National Resource for Imaging Mass Spectroscopy, Harvard Medical School
  • 2001-2009: Chairman, Advisory Committee National Resource for Imaging Mass Spectroscopy, Harvard Medical School
  • 1997-2001: Consultant Molecular Simulations Inc., San Diego CA
  • 1997-1999: Consultant Aurora Biosciences, La Jolla, CA
  • 1988-1998: Consultant LIDAK Pharmacuticals, La Jolla, CA
  • 1987-1988: Consultant Quidel Pharmacuticals, La Jolla, CA
  • 1972: Consultant, Nuclear Structure Laboratory, YaleUniversity
  • 1970: Guest Scientist, Nuclear Physics Laboratory, Weizman Institute, Israel

Honors and Awards

  1. 1982-1987: Established Investigatorship of the American Heart Association
  2. 1979: American Cancer Society Junior Faculty Award
  3. 1976-1979: National Research Service Award (NIH)

Selected Publications

Selected Publications (10 of 102)

  1. Kampf, J. P., and Kleinfeld, A. M. Fatty acid transport in adipocytes monitored by imaging intracellular FFA levels. J. Biol. Chem., 279: 35775- 35780, 2004.
  2. Kleinfeld, A. M., Kampf, J. P., and Lechene, C. Transport of 13C-oleate in adipocytes measured using multi imaging mass spectrometry. J. Am. Soc. Mass Spectrom., 15: 1572-1580, 2004.
  3. Yuvienco, J.M., Dizon, E. C., Kleinfeld, A. M., Anwar, M., Hiatt, M., and Hegyi, T., Umbilical cord unbound free fatty acid concentration and low Apgar score. Am. J. Perinatology,22: 429-36, 2005
  4. Kleinfeld, A. M., and Okada, C., Free fatty acid release from human breast cancer tissue inhibits cytotoxic T lymphocyte- mediated killing. J. Lipid Res., 46:1983-1990, 2005
  5. Huber, A., Kampf, J. P., Kwan, T. Zhu, B. and Kleinfeld, A. M., Fatty acid-specific fluorescent probes and their use in resolving mixtures of different unbound free fatty acids in equilibrium with albumin. Biochemistry, 45:14263-14274, 2006
  6. Lechene, C., Hillion, F., McMahon, G., Benson, D., Kleinfeld, A. M., Kampf, J. P., Daniel, D., Luyten, Y., Bonventre, J., Hentschel, D., Park, K., M., Ito, S., Schwartz, M., Benichou, G., Slodzian, G. High-resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry. J. Biol., 5:20.0-20.30, 2006
  7. Kampf, J. P. and Kleinfeld, A. M., Membrane transport of FFA mediated by lipid, protein, or both? – Point/Counterpoint. Physiology, 22:7-14, 2007.
  8. Kampf, J. P., Parmley, D. and Kleinfeld, A. M., Free fatty acid transport across adipocytes is mediated by an unknown membrane protein pump. Am. J. Physiology Endocrinol Metab, 293:E1207-E1214, 2007.
  9. Cantor, W. J., Hoe-Kim, H., Jolly S., Moe, G., Burstein, J. M., Mendelsohn A., Kleinfeld, A. M., and Fitchett, D., B-Type Natriuretic Peptide and Serum Unbound Free Fatty Acid Levels after Contemporary Percutaneous Coronary Intervention. J. Invasive Cardiology, 29:186-188, 2008.
  10. Carley, A. N. and Kleinfeld, A. M., Flip-Flop Is the Rate-Limiting Step for Transport of Free Fatty Acids across Lipid Vesicle Membranes. Biochemistry 48:10437-10445, 2009.

Patents

Patents (5 of 7)

  1. One Step Free Fatty Acid Determination (U.S. Patent No. 5,470,714, Eur. Patent No. 0457901) and Hong Kong
  2. Method of Detection of Cardiac Ischemia Using Fatty Acid Binding Protein (US Patent No. 6,444,432 B1)
  3. Method of detection of cardiac ischemia using fatty acid binding proteins (US Patent No. 6,750,030 B2)
  4. Method Method and apparatus for ratio fluorometry (US Patent No. 6,999,173 B2)
  5. Early diagnosis of stroke (US Patent No. 7,202,089) also Canada, Europe, China